Evaluation of the Cholestech LDX

Evaluation of the Cholestech L.D.X
Lipid Analyzer for Cholesterol,
Triglyceride and HDL Cholesterol (HDL-C)
Using Whole Blood

Abstract Submitted to
AACC, American Association of Clinical Chemistry
July, 1993

Carrie Carlson, Pamela Roberts and Ronald Feld
Department of Pathology, University of lowa Hospitals
lowa City, lowa

The Cholestech LDX Analyzer (Cholestech Corporation, Hayward, CA) performs cholesterol, triglyceride, and HDL-C using a single dry reagent cassette on 35 ul of whole blood in four minutes We compared the LDX to analyses performed on the Hitachi 717 and 747 (BMD Diagnostics, Indianapolis, IN) using both EDTA and heparinized plasma We also compared the LDX to analyses performed in the Lipid Laboratory using EDTA plasma analyzed on the Abbott Spectrum (Abbott Diagnostics, Abbott Park, IL) HDL-C was performed using Mg phosphotungstic acid on the Hitachi 747 and Mg Dextran sulfate on the LDX and Abbott Spectrum Linear regression slope and intercept data performed on 50 fasting samples (triglycerides = 41 ) was:

Hitachi EDTA (X)
105, 70
0.94, 5.0
1.09, 1.1
Hitachi Heparin (X)
0.99, 10.1
0.88, 6.6
Abbott EDTA (X)
0.97, 8.6
0.88, 4.0
1.04, 7.9

Total imprecision for two levels of control material ranged from 3.3 % to 3.6 % for cholesterol,
5 1% to 5 7% for triglycerides, and 4 4% to 8.6 % for HDL-C.

The CLA LDX performs accurate, precise, and rapid lipid analysis on whole blood.